| Wu A |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=3.643 ------>paper_class3=2 ------>paper_class2=1 ------>vol=213 ------>confirm_bywho=None ------>insert_bywho=chaw1211 ------>Jurnal_Rank=28.2 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=211 ------>medlineContent= ------>unit=000 ------>insert_date=20081211 ------>iam=1 ------>update_date=None ------>author=??? ------>change_event=1 ------>ISSN= ------>authors_c= ------>score=500 ------>journal_name=J Cell Physiol ------>paper_name=Sperm surface arylsulfatase A can disperse the cumulus matrix of cumulus oocyte complexes ------>confirm_date=None ------>tch_id=097004 ------>pmid=17474085 ------>page1=201 ------>fullAbstract=Cumulus cell layers of expanded cumulus oocyte complexes (COCs) are interlinked with networks of hyaluronic acid, chondroitin sulfate B proteoglycans and link proteins, and they can be dispersed by sperm surface hyaluronidases. In this report, we showed that arylsulfatase A (AS-A), existing on the sperm head surface, also had this dispersion action. Purified AS-A free of protease, hyaluronidase and chondroitinase activities could disperse the cumulus matrix of expanded COCs. However, this COC dispersion action was not associated with AS-A desulfation activity, assayed by using p-nitrocatecholsulfate (artificial substrate). COCs incubated for 1 h with sperm pretreated with anti-AS-A IgG in the presence of apigenin (a hyaluronidase inhibitor) did not exhibit matrix dispersion, whereas several cumulus layers were already dispersed in COCs incubated with sperm pretreated with preimmune IgG. Furthermore, sperm from AS-A null mice showed a significant delay in COC dispersion, compared with wild-type sperm. Within 1 h of sperm-COC co-incubation, the size of COCs incubated with AS-A null sperm was 65% of the original dimension, whereas that of COCs inseminated with wild-type sperm was only 17%. A further delay in COC dispersion by AS-A(-/-) mouse sperm was observed when apigenin was present in the co-incubation. We also showed for the first time that AS-A had a specific affinity for chondroitin sulfate B, a component of cumulus matrix proteoglycan networks; this might provide a mechanism of cumulus matrix destabilization induced by sperm surface AS-A. ------>tmu_sno=None ------>sno=20714 ------>authors2=Anupriwan A ------>authors3=Iamsaard S ------>authors4=Charkrabandhu K ------>authors5=Santos DC ------>authors6=Rupar T, Tsang BK, Carmona E, Tanphaichitr N ------>authors6_c= ------>authors=Wu A ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Sperm surface arylsulfatase A can disperse the cumulus matrix of cumulus oocyte complexes. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no=1 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2007 ------>submit_flag=None ------>publish_month=10 |