| Wu AT |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=5.581 ------>paper_class3=2 ------>paper_class2=1 ------>vol=282 ------>confirm_bywho=None ------>insert_bywho=chaw1211 ------>Jurnal_Rank=15.2 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=20 ------>paper_class2Letter=None ------>page2=12175 ------>medlineContent= ------>unit=000 ------>insert_date=20081211 ------>iam=1 ------>update_date=None ------>author=??? ------>change_event=1 ------>ISSN= ------>authors_c= ------>score=500 ------>journal_name=J Biol Chem ------>paper_name=PAWP, a sperm-specific WW domain-binding protein, promotes meiotic resumption and pronuclear development during fertilization ------>confirm_date=None ------>tch_id=097004 ------>pmid=17289678 ------>page1=12164 ------>fullAbstract=We report a novel alkaline extractable protein of the sperm head that exclusively resides in the post-acrosomal sheath region of the perinuclear theca (PT) and is expressed and assembled in elongating spermatids. It is a protein that shares sequence homology to the N-terminal half of WW domain-binding protein 2, while the C-terminal half is unique and rich in proline. A functional PPXY consensus binding site for group-I WW domain-containing proteins, and numerous unique repeating motifs, YGXPPXG, are identified in the proline-rich region. Considering these molecular characteristics, we designated this protein PAWP for postacrosomal sheath WW domain-binding protein. Microinjection of recombinant PAWP or alkaline PT extract into metaphase II-arrested porcine, bovine, macaque, and Xenopus oocytes induced a high rate of pronuclear formation, which was prevented by co-injection of a competitive PPXY motif containing peptide derived from PAWP but not by co-injection of the point-mutated peptide. Intracytoplasmic sperm injection (ICSI) of porcine oocytes combined with co-injection of the competitive PPXY peptide or an anti-recombinant PAWP antiserum prevented pronuclear formation and arrested fertilization. Conversely, co-injection of the modified PPXY peptide, when the tyrosine residue of PPXY was either phosphorylated or substituted with phenylalanine, did not prevent ICSI-induced fertilization. This study uncovers a group I WW domain module signal transduction event within the fertilized egg that appears compulsory for meiotic resumption and pronuclear development during egg activation and provides compelling evidence that a PPXY motif of sperm-contributed PAWP can trigger these events. ------>tmu_sno=None ------>sno=20715 ------>authors2=Sutovsky P ------>authors3=Manandhar G ------>authors4=Xu W ------>authors5=Katayama M ------>authors6=Day BN, Park KW, Yi YJ, Xi YW, Prather RS, Oko R ------>authors6_c= ------>authors=Wu AT ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=PAWP, a sperm-specific WW domain-binding protein, promotes meiotic resumption and pronuclear development during fertilization. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no=16 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2007 ------>submit_flag=None ------>publish_month=4 |