| Yang LL |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=1.848 ------>paper_class3=2 ------>paper_class2=1 ------>vol=69 ------>confirm_bywho=amel ------>insert_bywho=llyang ------>Jurnal_Rank=27.6 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=1095 ------>medlineContent= ------>unit=G0100 ------>insert_date=20081215 ------>iam=1 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=500 ------>journal_name=Planta Med. ------>paper_name=Cytotoxic activity of coumarins from the fruits of Cnidium monnieri on leukemia cell lines ------>confirm_date=20081217 ------>tch_id=058001 ------>pmid=14750023 ------>page1=1091 ------>fullAbstract=Cnidii monnieri Fructus [CmF; Cnidium monnieri (L.) Cusson] is used as a tonic agent in traditional Chinese medicine. In a previous Chinese herb-cytotoxicity screening test, the ethanol extract of CmF exhibited strong effects on human leukemia (HL-60), cervical carcinoma (HeLa) and colorectal carcinoma (CoLo 205) cells. Then, the CmF extract was subjected to silica gel column chromatography and recrystallization to give five coumarins: osthol, imperatorin, bergapten, isopimpinellin, and xanthotoxin. Among these compounds, osthol showed the strongest cytotoxic activity on tumor cell lines. The structure-activity relationship established from the results indicated that the prenyl group has an important role in the cytotoxic effects. However, imperatorin showed the highest sensitivity to HL-60 cells and the least cytotoxicity to normal PBMCs. Osthol and imperatorin both caused apoptotic bodies, DNA fragmentation, and enhanced PARP degradation in HL-60 cells by biochemical analysis. These results indicate that osthol and imperatorin can induce apoptosis in HL-60 cells. Therefore, osthol and imperatorin are cytotoxic marker substances in the fruits of Cnidium monnieri. ------>tmu_sno=None ------>sno=20843 ------>authors2=Wang MC ------>authors3=Chen LG ------>authors4=Wang CC ------>authors5= ------>authors6= ------>authors6_c= ------>authors=Yang LL ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Cytotoxic activity of coumarins from the fruits of Cnidium monnieri on leukemia cell lines. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no=12 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2003 ------>submit_flag=None ------>publish_month=12 |