Hwang, L.L. and Dun, N.J. |
------>authors3_c=None ------>paper_class1=1 ------>Impact_Factor=None ------>paper_class3=2 ------>paper_class2=1 ------>vol=517 ------>confirm_bywho=wslee ------>insert_bywho=llhwang ------>Jurnal_Rank=None ------>authors4_c=None ------>comm_author= ------>patent_EDate=None ------>authors5_c=None ------>publish_day=None ------>paper_class2Letter=None ------>page2=228 ------>medlineContent= ------>unit=000 ------>insert_date=20000626 ------>iam=1 ------>update_date= ------>author=??? ------>change_event=5 ------>ISSN=None ------>authors_c=None ------>score=413 ------>journal_name=J. Physiol. ------>paper_name=5-hydroxytryptamine modulates multiple conductances in immature rat rostral ventrolateral medulla neurons in vitro. ------>confirm_date=20020507 ------>tch_id=089017 ------>pmid=10226161 ------>page1=217 ------>fullAbstract=1. Whole-cell patch-clamp recordings were made from rostral ventrolateral medulla (RVLM) neurones of brainstem slices from 8- to 12-day-old rats. In the presence of tetrodotoxin (0.5 microM), 5-HT (50 microM) elicited an outward current (I5-HT,outward) (10/44 neurones) associated with an increase in membrane conductance, and an inward current (I5-HT,inward) (29/44 neurones) accompanied by a decrease or no significant change in membrane conductance. 2. The steady-state I-V relationship of I5-HT,outward showed an inward rectification; the 5-HT-induced current, which reversed at -87.9 +/- 3.0 mV, was suppressed by 0.1 mM Ba2+. 3. Two types of steady-state I-V relationship for I5-HT,inward were noted: type I I5-HT,inward was characterized by a significant decrease in membrane conductance and reversed at a potential close to or negative to the theoretical K+ equilibrium potential (EK), -94 mV, in 8/17 neurones; type II I5-HT,inward was not associated with a significant change in membrane conductance and was relatively independent of membrane potential. 4. Both type I and type II I5-HT,inward were significantly reduced in a low [Na+]o solution. In this solution, I5-HT,inward decreased with hyperpolarization and had a linear steady-state I-V relationship with a reversal potential of approximately -110 mV. The reversal potential of type I I5-HT,inward shifted to about -80 mV as the [K+]o was increased from 3.1 to 7.0 mM in low [Na+]o solution. The type II I5-HT,inward did not reverse at the estimated EK in the same solution. 5. While not affected by externally applied Cs+ (1 mM), I5-HT,inward was significantly smaller in RVLM neurones patched with Cs+-containing electrodes; the current reversed at -11.9 +/- 6.4 mV in 8/15 responsive neurones. 6. It may be concluded that in rat RVLM neurones 5-HT increases an inwardly rectifying K+ conductance which may underlie the I5-HT, outward and that a combination of varying degrees of K+ conductance decrease and a Cs+-insensitive, non-selective cation conductance increase may account for the two types of conductance change associated with I5-HT,inward. ------>tmu_sno=None ------>sno=2095 ------>authors2=None ------>authors3=None ------>authors4=None ------>authors5=None ------>authors6=None ------>authors6_c=None ------>authors=Hwang, L.L. and Dun, N.J. ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c=None ------>publish_area=None ------>updateTitle=5-HT modulates multiple conductances in immature rat rostral ventrolateral medulla neurones in vitro. ------>language=2 ------>check_flag= ------>submit_date= ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho= ------>publish_year=1999 ------>submit_flag= ------>publish_month=None |