| Cheng KC |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=4.180 ------>paper_class3=2 ------>paper_class2=1 ------>vol=8 ------>confirm_bywho=None ------>insert_bywho=jhchen ------>Jurnal_Rank=15.2 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=421 ------>medlineContent= ------>unit=000 ------>insert_date=20090306 ------>iam=3 ------>update_date=None ------>author=??? ------>change_event=2 ------>ISSN= ------>authors_c= ------>score=500 ------>journal_name=BMC Genomics ------>paper_name=Ganoderma lucidum polysaccharides in human monocytic leukemia cells: from gene expression to network construction. ------>confirm_date=None ------>tch_id=097132 ------>pmid=17996095 ------>page1=411 ------>fullAbstract=BACKGROUND: Ganoderma lucidum has been widely used as a herbal medicine for promoting health and longevity in China and other Asian countries. Polysaccharide extracts from Ganoderma lucidum have been reported to exhibit immuno-modulating and anti-tumor activities. In previous studies, F3, the active component of the polysaccharide extract, was found to activate various cytokines such as IL-1, IL-6, IL-12, and TNF-alpha. This gave rise to our investigation on how F3 stimulates immuno-modulating or anti-tumor effects in human leukemia THP-1 cells. RESULTS: Here, we integrated time-course DNA microarray analysis, quantitative PCR assays, and bioinformatics methods to study the F3-induced effects in THP-1 cells. Significantly disturbed pathways induced by F3 were identified with statistical analysis on microarray data. The apoptosis induction through the DR3 and DR4/5 death receptors was found to be one of the most significant pathways and play a key role in THP-1 cells after F3 treatment. Based on time-course gene expression measurements of the identified pathway, we reconstructed a plausible regulatory network of the involved genes using reverse-engineering computational approach. CONCLUSION: Our results showed that F3 may induce death receptor ligands to initiate signaling via receptor oligomerization, recruitment of specialized adaptor proteins and activation of caspase cascades. ------>tmu_sno=None ------>sno=21197 ------>authors2=Huang HC ------>authors3=Chen JH ------>authors4=Hsu JW ------>authors5=Cheng HC ------>authors6=Ou CH, Yang WB, Chen ST, Wong CH , Juan HF ------>authors6_c= ------>authors=Cheng KC ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Ganoderma lucidum polysaccharides in human monocytic leukemia cells: from gene expression to network construction. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2007 ------>submit_flag=None ------>publish_month=1 |