Cheng MF |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=1.074 ------>paper_class3=2 ------>paper_class2=1 ------>vol=61 ------>confirm_bywho=ncchang ------>insert_bywho=yungching ------>Jurnal_Rank=94.0 ------>authors4_c= ------>comm_author=1 ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=24 ------>medlineContent= ------>unit=E0109 ------>insert_date=20090312 ------>iam=7 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=464 ------>journal_name=Jpn J Infect Dis ------>paper_name=Clinical application of reverse-transcription polymerase reaction and intravenous immunoglobulin for enterovirus encephalitis. ------>confirm_date=20090312 ------>tch_id=097070 ------>pmid=18219129 ------>page1=18 ------>fullAbstract=Although polymerase chain reaction (PCR) is a highly sensitive procedure for the diagnosis of enteroviruses, it has never been systemically applied to the treatment of enteroviral encephalitis using intravenous immunoglobulin (IVIg). We conducted a 2-year randomized, controlled comparison of reverse transcription (RT)-PCR of cerebrospinal fluid (CSF) with traditional viral isolation to guide IVIg treatment. Seventy-five patients were enrolled and classified into three groups: one group with clinical manifestations of enteroviral infections and two without. The latter two groups were separated on the basis of whether IVIg treatment was guided by RT-PCR or virus culture assay. CSF specimens from the 18 confirmed cases of enteroviral encephalitis were RT-PCR positive for enterovirus in all but one case. Of the remaining 57 cases of nonenteroviral encephalitis, only 4 were positive for enterovirus RT-PCR. One patient in the group of IVIg treatment guided by viral isolation subsequently displayed a sequel of epilepsy. No patients in the IVIg treatment groups guided by RT-PCR had any neurological sequelae. In conclusion, the use of RT-PCR allowed rapid, sensitive, and specific detection of enteroviral RNA in CSF. When used to guide IVIg treatment, RT-PCR may shorten hospitalization and improve outcomes of patients with enteroviral encephalitis. ------>tmu_sno=None ------>sno=21439 ------>authors2=Chen BC ------>authors3=Huang KS ------>authors4=Chen SN ------>authors5=Liu YC ------>authors6= ------>authors6_c= ------>authors=Cheng MF ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Clinical application of reverse-transcription polymerase chain reaction and intravenous immunoglobulin for enterovirus encephalitis. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2008 ------>submit_flag=None ------>publish_month=1 |