| Wei-Hwa Lee |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=3.591 ------>paper_class3=2 ------>paper_class2=1 ------>vol=0 ------>confirm_bywho=None ------>insert_bywho=whl95816 ------>Jurnal_Rank=30.3 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=0 ------>medlineContent= ------>unit=J0600 ------>insert_date=20090313 ------>iam=1 ------>update_date=None ------>author=??? ------>change_event=2 ------>ISSN= ------>authors_c=??? ------>score=500 ------>journal_name=Journal of Cellular Biochemistry ------>paper_name=JAK pathway induction of c-Myc critical to IL-5 stimulation of cell proliferation and inhibition of apoptosis ------>confirm_date=None ------>tch_id=095048 ------>pmid=19180571 ------>page1=0 ------>fullAbstract=Interleukin-5 (IL-5) induction of c-Myc expression is associated with IL-5 inhibition of apoptosis in hematopoietic cells. In this study, TFalpha1 and TFalpha8 cells with stable overexpression of IL-5 receptor alpha (IL-5Ralpha) subunit in TF-1 cells, a human hematopoietic progenitor cell line which expressed low levels of IL-5Ralpha, were used to explored how IL-5 up-regulate c-Myc and the role of c-Myc in IL-5 signaling. First, we demonstrate that IL-5 induced c-Myc RNA and protein expressions, as well as activated Janus kinases (JAK1 and JAK2) and signal transducer and activator of transcription-5b (STAT5b). JAK inhibitor AG490 and c-Myc inhibitor 10058-F4, both, reduced IL-5-mediated cell proliferation in a dose- and time-dependent manner. Both, AG490 and 10058-F4, also reduced IL-5-mediated anti-apoptotic activity. Furthermore, AG490 inhibited IL-5-mediated c-Myc induction and promoter activity. We further examined the role of JAK1 and JAK2 in the induction of c-Myc expression using the CDJAK fusion proteins, which consisted of a CD16 extracellular domain, a CD7 transmembrane domain, and either JAK1 (CDJAK1) or JAK2 (CDJAK2) as intracellular domains. Simultaneous activation of JAK1 and JAK2 by anti-CD16 antibody crosslinking of CDJAK1 and CDJAK2 could induced c-Myc expression and promoter activity; AG490 inhibited CDJAK1 and CDJAK2-mediated effects. These results suggest that IL-5 induces cell proliferation and anti-apoptosis through the JAK/c-Myc pathway, and that JAK1 and JAK2 activation participate in IL-5-induced up-regulation of c-Myc. ------>tmu_sno=None ------>sno=21495 ------>authors2=Fu-Hwa Liu ------>authors3=John Yi-Chung Liv ------>authors4=Shin-Yun Huang ------>authors5=Heng Lin ------>authors6=Wei-Ju Liao,Huei-Mei Huang ------>authors6_c=??? ------>authors=Wei-Hwa Lee ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=JAK pathway induction of c-Myc critical to IL-5 stimulation of cell proliferation and inhibition of apoptosis. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no=0 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2009 ------>submit_flag=None ------>publish_month=6 |