| Su SL |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=3.793 ------>paper_class3=2 ------>paper_class2=1 ------>vol=13 ------>confirm_bywho=shiemin ------>insert_bywho=lee060008 ------>Jurnal_Rank=2.1 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=886 ------>medlineContent= ------>unit=E0116 ------>insert_date=20090318 ------>iam=3 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=500 ------>journal_name=Osteoarthritis Cartilage ------>paper_name=Expression and regulation of Toll-like receptor 2 by IL-1beta and fibronectin fragments in human articular chondrocytes ------>confirm_date=20090318 ------>tch_id=097107 ------>pmid=15961329 ------>page1=879 ------>fullAbstract=OBJECTIVE: The objective of this study was to examine expression and regulation of Toll-like receptor 2 (TLR2) in human articular chondrocytes. METHODS: Human articular chondrocytes were enzymatically isolated from normal and osteoarthritic knee cartilage. Immunohistochemistry, Western blotting, and reverse transcriptase-polymerase chain reaction (RT-PCR) were used to assess the expression of toll-like receptors. Following stimulation of chondrocytes in vitro by IL-1beta and fibronectin proteolytic fragments, the relative levels of mRNA for TLR2 were determined by quantitative real-time PCR. MyD88 activation and nuclear factor-kappaB (NF-kappaB) translocation were evaluated by immunoprecipitation and electrophoretic mobility shift assay, respectively. RESULTS: Human articular chondrocytes mainly expressed TLR1, 2, 5 by RT-PCR. Protein expression of TLR2 was also identified in adult human articular cartilage. TLR2 was upregulated following IL-1beta and fibronectin proteolytic fragments stimulation in primary cultures of osteoarthritic articular chondrocytes. Fibronectin proteolytic fragments-induced TLR2 upregulation involved an IL-1beta autocrine/paracrine pathway. CONCLUSIONS: TLR2 is expressed in human articular cartilage and is upregulated by proarthritic agents including IL-1beta and fibronectin fragments. Signaling through TLR is a novel pro-inflammatory mechanism in osteoarthritis and targeting of these signaling pathways may be of value in treatment of degenerative joint disease. ------>tmu_sno=None ------>sno=21659 ------>authors2=Tsai CD ------>authors3=Lee CH ------>authors4=Salter DM ------>authors5=Lee HS ------>authors6= ------>authors6_c= ------>authors=Su SL ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Expression and regulation of Toll-like receptor 2 by IL-1beta and fibronectin fragments in human articular chondrocytes. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no=10 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2005 ------>submit_flag=None ------>publish_month=10 |