| Chan, A L-F |
------>authors3_c=??? ------>paper_class1=1 ------>Impact_Factor=0.940 ------>paper_class3=2 ------>paper_class2=1 ------>vol=14 ------>confirm_bywho=cmshih ------>insert_bywho=cmlin ------>Jurnal_Rank=69.6 ------>authors4_c=??? ------>comm_author=1 ------>patent_EDate=None ------>authors5_c=??? ------>publish_day=27 ------>paper_class2Letter=None ------>page2=1352 ------>medlineContent= ------>unit=E0103 ------>insert_date=20090330 ------>iam=7 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN=1420-3049 ------>authors_c=??? ------>score=500 ------>journal_name=Molecules ------>paper_name=Evodiamine stabilizes topoisomerase I-DNA cleavable complex to inhibit topoisomerase I activity ------>confirm_date=20090728 ------>tch_id=081006 ------>pmid=19384267 ------>page1=1342 ------>fullAbstract=Evodiamine (EVO), an alkaloidal compound isolated from Evodia rutaecarpa (Juss.), has been reported to affect many physiological functions. Topoisomerase inhibitors have been developed in a variety of clinical applications. In the present study, we report the topoisomerase I (TopI) inhibitory activity of EVO, which may have properties that lead to improved therapeutic benefits. EVO is able to inhibit supercoiled plasmid DNA relaxation catalyzed by TopI. Upon treatment 0-10 microM EVO TopI was depleted in MCF-7 breast cancer cells in a concentration-dependent and time-dependent manner in 0-120 min. A K-SDS precipitation assay was performed to measure the extent of Top I-trapped chromosomal DNA. The ability of EVO to cause the formation of a TopI-DNA complex increased in a concentration-dependent manner, in that the DNA trapped increased by 24.2% in cells treated with 30 microM. The results suggest that EVO inhibits TopI by stabilizing the enzyme and DNA covalent complex. ------>tmu_sno=None ------>sno=21741 ------>authors2=Chang WS ------>authors3=Chen LM ------>authors4=Lee CM ------>authors5=Chen CE ------>authors6=Lin CM, Hwang J ------>authors6_c=???, ??? ------>authors=Chan, A L-F ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c=??? ------>publish_area=0 ------>updateTitle=Evodiamine stabilizes topoisomerase I-DNA cleavable complex to inhibit topoisomerase I activity. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2009 ------>submit_flag=None ------>publish_month=3 |