| Chang CC |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=5.511 ------>paper_class3=2 ------>paper_class2=1 ------>vol=66 ------>confirm_bywho=hsuan ------>insert_bywho=juansh ------>Jurnal_Rank=15.2 ------>authors4_c= ------>comm_author=1 ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=205 ------>medlineContent= ------>unit=E0105 ------>insert_date=20090731 ------>iam=7 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=500 ------>journal_name=Cell Mol Life Sci. 2009 ------>paper_name=Aryl-hydrocarbon receptor-dependent alteration of FAK/RhoA in the inhibition of HUVEC motility by 3-methylcholanthrene. ------>confirm_date=20091014 ------>tch_id=090103 ------>pmid=19649566 ------>page1=3193 ------>fullAbstract=We previously demonstrated the antiproliferative and antiangiogenic effects of 3-methylcholanthrene (3MC), an aryl-hydrocarbon receptor (AhR) agonist, in human umbilical vascular endothelial cells (HUVECs). Herein, we unraveled its molecular mechanisms in inhibiting HUVEC motility. 3MC down-regulated FAK, but up-regulated RhoA, which was rescued by AhR knockdown. It led us to identify novel AhR binding sites in the FAK/RhoA promoters. Additionally, 3MC increased RhoA activity via suppression of a negative feedback pathway of FAK/p190RhoGAP. With an increase in membrane-bound RhoA, subsequent stress fiber and focal adhesion complex formation was observed in 3MC-treated cells, and this was reversed by a RhoA inhibitor and AhR antagonists. Notably, these compounds significantly reversed 3MC-mediated anti-migration in a transwell assay. The in vitro findings were further confirmed using an animal model of Matrigel formation in Balb/c mice. Collectively, AhR~s genomic regulation of FAK/RhoA, together with RhoA activation, is ascribable to the anti-migration effect of 3MC in HUVECs. ------>tmu_sno=None ------>sno=22061 ------>authors2=Tsai SY ------>authors3=Lin H ------>authors4=Li HF ------>authors5=Lee YH ------>authors6=Chou Y, Jen CY, Juan SH ------>authors6_c= ------>authors=Chang CC ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Aryl-hydrocarbon receptor-dependent alteration of FAK/RhoA in the inhibition of HUVEC motility by 3-methylcholanthrene. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no=19 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2009 ------>submit_flag=None ------>publish_month=10 |