| Chou FF |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=4.104 ------>paper_class3=2 ------>paper_class2=1 ------>vol=20 ------>confirm_bywho=wslee ------>insert_bywho=cmbsfc21 ------>Jurnal_Rank=16.0 ------>authors4_c= ------>comm_author=1 ------>patent_EDate=None ------>authors5_c= ------>publish_day=9 ------>paper_class2Letter=0 ------>page2=1350 ------>medlineContent= ------>unit=E1300 ------>insert_date=20090805 ------>iam=3 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=500 ------>journal_name=Hum Gene Ther. ------>paper_name=Oral Gene Therapy for Hypoparathyroidism-a rat model. ------>confirm_date=20091112 ------>tch_id=083008 ------>pmid=19619000 ------>page1=1344 ------>fullAbstract=Abstract The use of nonionic polymeric micelles orally to protect and deliver plasmid DNA in vivo was investigated. Parathyroid hormone (PTH)(1-34) gene (179 bp) was inserted into a human cytomegalovirus promoter (PCMV) and E. coli competent cells were used to amplify the cDNA. Polymeric micelle formations (100 mul) formed from PCMV-PTH(1-34) cDNA (7.2 mug/mul) and 6% (w/v) polyethylene oxide-polypropylene oxide-polyethylene oxide (PEO-PPO-PEO) was administered at 8-hr intervals for 48 hr and then at 8-hr intervals for 24 hr weekly for 3 weeks. Parathyroidectomized rats receiving 150 mul of EDTA (10 mM) before each dose of formation served as the study group; rats receiving drinking water, EDTA (10 mM), PCMV-PTH(1-34) cDNA and PCMV-PTH(1-34) cDNA plus EDTA at the same amount and time intervals served as the control groups. Serum levels of calcium and PTH(1-34) were measured weekly for 4 weeks. Immunohistochemical stain for PTH(1-34), reverse transcriptase polymerase chain reaction for PTH(1-34) mRNA and the relative density of PTH(1-34) mRNA were performed at 2 and 4 weeks after oral gene therapy in different organs. One third to three of five rats in the control groups died after parathyroidectomy. Serum levels of calcium and PTH(1-34) were higher in the study than in the control groups. In the study group, positive stain of PTH(1-34) and PTH(1-34) mRNA could be found in those organs. Relative densities of PTH(1-34) mRNA were higher in the study than in the drinking water group in different organs. Oral gene therapy can maintain calcium and PTH(1-34) levels in parathyroidectomized rats. ------>tmu_sno=None ------>sno=22077 ------>authors2=Huang SC ------>authors3=Chang SF ------>authors4=Liaw J. ------>authors5=Hung PH ------>authors6= ------>authors6_c= ------>authors=Chou FF ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Oral gene therapy for hypoparathyroidism: a rat model. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2009 ------>submit_flag=None ------>publish_month=10 |