| Lee KH |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=7.216 ------>paper_class3=2 ------>paper_class2=1 ------>vol= ------>confirm_bywho=lm ------>insert_bywho=yehsd ------>Jurnal_Rank=10.5 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=13 ------>paper_class2Letter=None ------>page2= ------>medlineContent= ------>unit=E0117 ------>insert_date=20090902 ------>iam=3 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=495 ------>journal_name=Oncogence ------>paper_name=MicroRNA-330 acts as tumor suppressor and induces apoptosis of prostate cancer cells through E2F1-mediated suppression of Akt phosphortlation ------>confirm_date=20090902 ------>tch_id=091054 ------>pmid=19597470 ------>page1= ------>fullAbstract=MicroRNAs (miRNAs) make up a novel class of gene regulators; they function as oncogenes or tumor suppressors by targeting tumor-suppressor genes or oncogenes. A recent study that analysed a large number of human cancer cell lines showed that miR-330 is a potential tumor-suppressor gene. However, the function and molecular mechanism of miR-330 in determining the aggressiveness of human prostate cancer has not been studied. Here, we show that miR-330 is significantly lower expressed in human prostate cancer cell lines than in nontumorigenic prostate epithelial cells. Bioinformatics analyses reveal a conserved target site for miR-330 in the 3~-untranslated region (UTR) of E2F1 at nucleotides 1018-1024. MiR-330 significantly suppressed the activity of a luciferase reporter containing the E2F1-3~-UTR in the cells. This activity could be abolished with the transfection of anti-miR-330 or mutated E2F1-3~-UTR. In addition, the expression level of miR-330 and E2F1 was inversely correlated in cell lines and prostate cancer specimens. After overexpressing of miR-330 in PC-3 cells, cell growth was suppressed by reducing E2F1-mediated Akt phosphorylation and thereby inducing apoptosis. Collectively, this is the first study to show that E2F1 is negatively regulated by miR-330 and also show that miR-330 induces apoptosis in prostate cancer cells through E2F1-mediated suppression of Akt phosphorylation. ------>tmu_sno=None ------>sno=22147 ------>authors2=Chen YL ------>authors3=Yeh SD ------>authors4=Hsiao M ------>authors5=Lin JT ------>authors6=Goan YG,Lu PJ ------>authors6_c= ------>authors=Lee KH ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=MicroRNA-330 acts as tumor suppressor and induces apoptosis of prostate cancer cells through E2F1-mediated suppression of Akt phosphorylation. ------>language=1 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2009 ------>submit_flag=None ------>publish_month=7 |