| Hu CM |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=4.902 ------>paper_class3=2 ------>paper_class2=1 ------>vol=130 ------>confirm_bywho=sylin ------>insert_bywho=sunpowerhu ------>Jurnal_Rank=8.3 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=306 ------>medlineContent= ------>unit=E0125 ------>insert_date=20090917 ------>iam=1 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=459 ------>journal_name=British J. Pharmacology ------>paper_name=Induction of skeletal contractures and calcium release from isolated sarcoplasmic reticulum vesicles by sanguinarine ------>confirm_date=20090918 ------>tch_id=098012 ------>pmid=10807666 ------>page1=299 ------>fullAbstract=The benzophenanthrine alkaloid, sanguinarine, was studied for its effects on isolated mouse phrenic-nerve diaphragm preparations. Sanguinarine induced direct, dose-dependent effects on muscle contractility. Sanguinarine-induced contracture was partially inhibited when the extracellular Ca(2+) was removed or when the diaphragm was pretreated with nifedipine. Depletion of sarcoplasmic reticulum (SR) internal calcium stores completely blocked the contracture. Sanguinarine induced Ca(2+) release from the actively loaded SR vesicles was blocked by ruthenium red and dithiothreitol (DTT), consistent with the ryanodine receptor (RyR) as the site of sanguinarine action. Sanguinarine altered [(3)H]-ryanodine binding to the RyR of isolated SR vesicles, potentiating [(3)H]-ryanodine binding at lower concentrations and inhibiting binding at higher concentrations. All of these effects were reversed by DTT, suggesting that sanguinarine-induced Ca(2+) release from SR occurs through oxidation of critical SH groups of the RyR SR calcium release channel. ------>tmu_sno=None ------>sno=22191 ------>authors2=Cheng HW ------>authors3=Cheng YW ------>authors4=Kang JJ ------>authors5= ------>authors6= ------>authors6_c= ------>authors=Hu CM ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Induction of skeletal muscle contracture and calcium release from isolated sarcoplasmic reticulum vesicles by sanguinarine. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2000 ------>submit_flag=None ------>publish_month=1 |