Taipei Medical University

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
Der-Zen Liu
------>authors3_c=
------>paper_class1=1
------>Impact_Factor=4.893
------>paper_class3=2
------>paper_class2=1
------>vol=1773
------>confirm_bywho=chenchho
------>insert_bywho=chenchho
------>Jurnal_Rank=19.6
------>authors4_c=
------>comm_author=
------>patent_EDate=None
------>authors5_c=
------>publish_day=1
------>paper_class2Letter=None
------>page2=879
------>medlineContent=
------>unit=E0310
------>insert_date=20090923
------>iam=3
------>update_date=None
------>author=???
------>change_event=4
------>ISSN=
------>authors_c=
------>score=500
------>journal_name=BBAmcr
------>paper_name=Switch activation of PI-PLC downstream signals in activated macrophages with wortmannin
------>confirm_date=20090924
------>tch_id=072003
------>pmid=17488650
------>page1=869
------>fullAbstract=Phosphatidylinositol (4,5)-bisphosphate (PtdIns(4,5)P(2)) has been known to serve as a substrate for phosphatidylinositol 3-kinase (PI(3)K) and phosphoinositide-specific phospholipase C (PI-PLC), which can produce PtdIns(3,4,5)P(3) and inositol 1,4,5-trisphosphate (Ins(1,4,5)P(3)) and diacylglycerol (DAG), respectively. In this study, we elucidated the role of PI-PLC during the LPS-activated mouse macrophages RAW264.7 treated with PI(3)K inhibitor wortmannin. First, wortmannin treatment enhanced Ins(1,4,5)P(3) production and iNOS expression in LPS-activated macrophages. Inhibition of PI(3)K by p85 siRNA also showed an enhancement of iNOS expression. On the other hand, overexpression of PI(3)K by ras-p110 expression plasmid significantly decreased iNOS expression in LPS-activated macrophages. In addition, overexpression of wild-type or dominant-negative Akt expression plasmid did not affect the iNOS expression in LPS-activated macrophages. Second, treatment of PI-PLC inhibitor U73122 reversed the enhancement of iNOS expression, the increase of phosphorylation level of ERK, JNK and p38, and the increase of AP-1-dependent gene expression in wortmannin-treated and LPS-activated macrophages. However, NF-kappaB activity determined by EMSA assay and reporter plasmid assay did not change during LPS-activated macrophages with or without wortmannin. We propose that the inhibition of PI(3)K by wortmannin in mouse macrophages enhances the PI-PLC downstream signals, and subsequently increases the LPS induction of iNOS expression independently of Akt pathway.
------>tmu_sno=None
------>sno=22278
------>authors2=Hong-Jen Liang
------>authors3=Chien-Ho Chen
------>authors4=Shyr-Yi Lin
------>authors5=Wen-Bin Zhong
------>authors6=Feng-Ming Ho,Wen-Chi Hou,Jui-Lien-Lien Lo,Yuan-Soon Ho,Pei-Jung Lin,Ling-Fang Hung, Yu-Chih Liang
------>authors6_c=
------>authors=Der-Zen Liu
------>delete_flag=0
------>SCI_JNo=None
------>authors2_c=
------>publish_area=0
------>updateTitle=Switch activation of PI-PLC downstream signals in activated macrophages with wortmannin.
------>language=2
------>check_flag=None
------>submit_date=None
------>country=None
------>no=
------>patent_SDate=None
------>update_bywho=None
------>publish_year=2007
------>submit_flag=None
------>publish_month=3
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z