Taipei Medical University

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
Shih-Chang Hsu
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------>journal_name=J allergy Clin Immunol
------>paper_name=Antigen coupled with Lewis-x trisaccharides elicits potent immune responses in mice
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------>fullAbstract=BACKGROUND: Glycoproteins containing Lewis-x (Le(x)) trisaccharides are often associated with the host~s adaptive T(H)2-type immunity, but the mechanisms underlying the T(H)2-biased response are at present unclear. OBJECTIVE: The modulatory effect of Le(x) or its glycoconjugates on IgE/T(H)2 responses was investigated. METHODS: The levels of serum antibodies and cytokines were analyzed by means of ELISA, RT-PCR, or both. RESULTS: In C3H mice Le(x) coupled with BSA (Le(x)-BSA) elicited higher levels of specific IgE and IgG1, but not IgG2a, which were associated with increased levels of splenic T(H)2 cytokines when compared with those seen in BSA-sensitized mice. In BALB/c mice sensitized with Le(x)-BSA or Le(x) mixed with ovalbumin, significantly increased levels of specific IgE and IgG2a antibodies were found concomitant with reduced levels of serum IL-12p70. These effects were attenuated in IL-12-deficient BALB/c mice. Le(x) and an isomer, Le(y), but not other isomers, inhibited the production of LPS-induced IL-12p70, associated with a significant reduction of nuclear NF-kappaB, in bone marrow-derived dendritic cells from BALB/c mice, suggesting that Le(x)-induced suppression of IL-12p70 results in an enhanced T(H)2 response. The addition of mannan, a known ligand for dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin, abrogated the suppressive effect of Le(x) trisaccharides. CONCLUSION: These results provide evidence for a potential role of Le(x) trisaccharides in shaping the immune responses through, at least in part, its suppressive effect on IL-12p70 production. Considering the relative ubiquity of glycoproteins with Le(x) or similar oligosaccharides, including plant-derived (or food-derived) allergens, these findings might have a broad implication. CLINICAL IMPLICATIONS: The adjuvant activity of Le(x) trisaccharides might aid in vaccine design and might be important in determining the allergenicity of proteins containing this or other similar structures.
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------>authors2=Tsung-Hsien Tsai
------>authors3=Hirokazu Kawasaki
------>authors4=Chien-Ho Chen
------>authors5=Beverly Plunkett
------>authors6=Reiko T.Lee, Yuan C.Lee, Shau-Ku Huang.
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------>authors=Shih-Chang Hsu
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------>updateTitle=Antigen coupled with Lewis-x trisaccharides elicits potent immune responses in mice.
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------>publish_year=2007
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A B C D E F G H I J K L M N O P Q R S T U V W X Y Z