| Chwei-Jang Chiou |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=6.646 ------>paper_class3=2 ------>paper_class2=1 ------>vol=30 ------>confirm_bywho=wpdeng ------>insert_bywho=tonyliu ------>Jurnal_Rank=3.9 ------>authors4_c= ------>comm_author=1 ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=5868 ------>medlineContent= ------>unit=F0700 ------>insert_date=20090924 ------>iam=7 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=500 ------>journal_name=Biomaterials ------>paper_name=Mucoadhesive liposomes for intranasal immunization with an avian influenza virus vaccine in chickens ------>confirm_date=20090925 ------>tch_id=090026 ------>pmid=19608270 ------>page1=5862 ------>fullAbstract=The aim of this study was to characterize a nasally delivered bioadhesive liposome using an inactivated H5N3 virus as a model antigen. Bioadhesive liposomes were developed using tremella (T) or xanthan gum (XG) as the bioadhesive polysaccharide. Using chickens as the target animal, we evaluated whether delivery of a bioadhesive liposomal influenza vaccine via a mucosal site of infection could improve vaccine effectiveness. 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)- 2H-tetrazolium (MTS) cytotoxicity assays demonstrated that T, XG and liposomes were non toxic to chicken spleen macrophages. Enzyme-linked immunosorbent assay (ELISA) was used to determine the adjuvant effect of the bioadhesive liposomal-vaccines. Chickens immunized with a low dose (200 microL) of bioadhesive liposomal influenza vaccine had significantly higher mucosal and serum antibody levels (P<0.05). In addition, liposomes mixed with a low-viscosity bioadhesive gel used for nasal delivery resulted in superior antibody responses compared with liposomes mixed with a high-viscosity gel (P<0.05). This suggest that a low-viscosity gel mixed with liposomes is more suitable for nasal delivery, and that chickens elicit higher mucosal secretory immunoglobulin A (s-IgA) and serum IgG after two vaccinations. ------>tmu_sno=None ------>sno=22294 ------>authors2=Li-Ping Tseng ------>authors3=Ming-Chung Deng ------>authors4=Pei-Rong Jiang ------>authors5=Shang-Li Tasi ------>authors6=Tze-Wen Chung, Yi-You Huang, Der-Zen Liu ------>authors6_c= ------>authors=Chwei-Jang Chiou ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Mucoadhesive liposomes for intranasal immunization with an avian influenza virus vaccine in chickens. ------>language=1 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2009 ------>submit_flag=None ------>publish_month=1 |