| Chang YC |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=None ------>paper_class3=1 ------>paper_class2=1 ------>vol=56 ------>confirm_bywho=clin ------>insert_bywho=pichen ------>Jurnal_Rank=None ------>authors4_c= ------>comm_author=1 ------>patent_EDate=None ------>authors5_c= ------>publish_day=21 ------>paper_class2Letter=None ------>page2=99 ------>medlineContent= ------>unit=H0100 ------>insert_date=20090925 ------>iam=2 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN=0047-262x ------>authors_c=??? ------>score=-16 ------>journal_name=???? ------>paper_name=??????????DNR????? ------>confirm_date=20090928 ------>tch_id=087007 ------>pmid=19918367 ------>page1=94 ------>fullAbstract=This study aims to evaluate the multidrug resistance (MDR) reversal activity by magnetic nanoparticles of Fe(3)O(4) (MNPs-Fe(3)O(4)) and 5-bromotetrandrine (BrTet) MDR cell line K562/A02 solitarily or symphysially. The proliferation of K562 and K562/A02 cells and the cytotoxicity on peripheral blood mononuclear cells (PMBCs) were evaluated by MTT assay. Cellular accumulation of daunorubicin (DNR) was analyzed by flow cytometry. Real-time polymerase chain reaction and Western blotting analyses were performed to examine the mRNA and protein levels of mdr1, respectively. The results showed that the combination of MNPs-Fe(3)O(4) and BrTet with effective concentrations significantly increased cytotoxicity against MDR cell line K562/A02. Both BrTet and MNPs-Fe(3)O(4) increased the intracellular DNR accumulation in the K562/A02 cell line, and downregulated the level of mdr1 gene and expression of P-glycoprotein. Furthermore, the combination did not have significant cytotoxicity in PMBCs. We propose that MNPs-Fe(3)O(4) conjugated with DNR and BrTet probably have synergetic effects on MDR reversal. ------>tmu_sno=None ------>sno=22319 ------>authors2=Chang PC ------>authors3= ------>authors4= ------>authors5= ------>authors6= ------>authors6_c= ------>authors=Chang YC ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c=??? ------>publish_area=0 ------>updateTitle=Effect of magnetic nanoparticles of Fe(3)O(4) and 5-bromotetrandrine on reversal of multidrug resistance in K562/A02 leukemic cells. ------>language=1 ------>check_flag=None ------>submit_date=None ------>country=None ------>no=4 ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2009 ------>submit_flag=None ------>publish_month=6 |