Taipei Medical University

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
Wei-Chih Cheng
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------>journal_name=Rapid Communications in Mass Spectrometry
------>paper_name=Determination of Urine Melamine by Validated Isotopic Ultra Performance Liquid Chromatography/ Tandem Mass Spectrometry. Rapid Communications in Mass Spectrometry
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------>fullAbstract=Little is known about melamine (MEL) analysis in children~s urine. In this study, an isotopic ultra-performance liquid chromatography/tandem mass spectrometry (UPLC/MS/MS) method was developed and systematically validated for the analysis of MEL in urine. The method is easily performed and comprises acidification, solid-phase extraction (SPE) and UPLC/MS/MS analysis. (13)C(3)N(3)((15)NH(2))(3) was used as the internal standard (IS) for calibration. Transition ions m/z 127 > 85 of MEL and m/z 133 > 89 of the IS were used for quantification and m/z 127 > 68 of MEL was used for quantitative confirmation. Recovery and precision were assessed to guarantee the applicability of the method. The limit of quantification (LOQ) was 0.01 microg/mL while the calculated method detection limit was 0.006 microg/mL. The mean recoveries ranged from 96-99%. The method was then applied to analyze urine samples from children who had potentially consumed MEL-tainted dairy products during screening in Taiwan. Ten nephrolithiasis cases and 20 age- and gender-matched controls were selected for this study. Three out of the 10 nephrolithiasis cases had elevated levels of MEL. Comparatively, twenty age- and gender-matched non-nephrolithiasis controls consuming Taiwan brand milk powder all showed MEL levels lower than the detection limit except for two children with background levels of 0.02 microg/mL. The background level in these children urine samples was established by UPLC/MS/MS analysis. Positive results of urine MEL tests might be associated with nephrolithiasis in these candidates. Measurement of urine MEL concentration can be helpful in confirming MEL-related nephrolithiasis, but its clinical application needs further clarification.
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------>authors3=Tien-Jen Lin
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------>authors=Wei-Chih Cheng
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------>updateTitle=Determination of urine melamine by validated isotopic ultra-performance liquid chromatography/tandem mass spectrometry.
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------>no=12
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------>publish_year=2009
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A B C D E F G H I J K L M N O P Q R S T U V W X Y Z