| Hu CM |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=2.260 ------>paper_class3=2 ------>paper_class2=1 ------>vol=121 ------>confirm_bywho=1 ------>insert_bywho=sunpowerhu ------>Jurnal_Rank=21.4 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=0 ------>page2=85 ------>medlineContent= ------>unit=E0125 ------>insert_date=20091109 ------>iam=1 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=500 ------>journal_name=Journal of Ethnopharmacology ------>paper_name=Heme oxygenase-1 mediates the inhibitory actions of brazilin in RAW264.7 macrophages stimulated with lipopolysaccharide ------>confirm_date=None ------>tch_id=098012 ------>pmid=18983903 ------>page1=79 ------>fullAbstract=Brazilin, the main constituent of Caesalpinia sappan L., is a natural red pigment that has been reported to possess anti-inflammatory properties. This study aimed to identify a novel anti-inflammatory mechanism of brazilin. We found that brazilin did not cause cytotoxicity below 300 microM, and activated heme oxygenase-1 (HO-1) protein synthesis in a concentration-dependent manner at 10-300 microM in RAW264.7 macrophages without affecting mRNA transcription of HO-1. Additionally, brazilin increased bilirubin production and HO-1 activity in RAW264.7 macrophages. In lipopolysaccharide (LPS)-stimulated macrophages, brazilin suppressed the release of nitric oxide (NO), prostaglandin E(2) (PGE(2)), interleukin (IL)-1beta and tumor necrosis factor-alpha (TNF-alpha), and reduced the expression of inducible nitric oxide synthase (iNOS). A specific inhibitor of HO-1, Zn(II) protoporphyrin IX, blocked the suppression of NO production, cytokines release and iNOS expression by brazilin. These results suggest that brazilin possesses anti-inflammatory actions in macrophages and works through a novel mechanism involving the action of HO-1. ------>tmu_sno=None ------>sno=22901 ------>authors2=Liua YH ------>authors3=Cheah KP ------>authors4=Li JS ------>authors5=Lam CSK ------>authors6=Lam CSK, Yu WY ------>authors6_c= ------>authors=Hu CM ------>delete_flag=0 ------>SCI_JNo=72298 ------>authors2_c= ------>publish_area=0 ------>updateTitle=Heme oxygenase-1 mediates the inhibitory actions of brazilin in RAW264.7 macrophages stimulated with lipopolysaccharide. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2009 ------>submit_flag=None ------>publish_month=6 |