Taipei Medical University

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
Deng, W.P., Hwang, J.J., Lin, G.L., Ngo, Q.H.
------>authors3_c=None
------>paper_class1=1
------>Impact_Factor=None
------>paper_class3=1
------>paper_class2=1
------>vol=5
------>confirm_bywho=curator
------>insert_bywho=ahom
------>Jurnal_Rank=None
------>authors4_c=None
------>comm_author=
------>patent_EDate=None
------>authors5_c=None
------>publish_day=None
------>paper_class2Letter=None
------>page2=223
------>medlineContent=
------>unit=F0400
------>insert_date=20010425
------>iam=1
------>update_date=
------>author=???
------>change_event=5
------>ISSN=None
------>authors_c=None
------>score=-30
------>journal_name=Therpeut Radiol Oncol.
------>paper_name=The gene regulation in cells by radiation.
------>confirm_date=20020207
------>tch_id=085041
------>pmid=19889963
------>page1=221
------>fullAbstract=Oxidative stress is one of the causative factors in the progression and etiology of age-related cataract. The Peroxiredoxin 6 (Prdx6), a savior for cells from internal or external environmental stresses, plays a role in cellular signaling by detoxifying reactive oxygen species (ROS) and thereby controlling gene regulation. Using targeted inactivation of the Prdx6 gene, here we show that Prdx6-deficient lens epithelial cells (LECs) are more vulnerable to UV-triggered cell death, a major cause of skin disorders including cataractogenesis, and these cells display abnormal protein profiles. PRDX6-depleted LECs showed phenotypic changes and formed lentoid body, a characteristic of terminal cell differentiation and epithelial mesenchymal transition (EMT). Prdx6(-/-) LECs exposed to UV-B showed higher ROS expression, and were prone to apoptosis compared to wild-type LECs, underscoring protective role for Prdx6. The comparative proteomic analysis using fluorescence-based difference gel electrophoresis along with Mass spectrometry and database searching revealed a total number of 13 proteins that were differentially expressed in Prdx6(-/-) cells. Six proteins were upregulated, where as expression of 7 proteins were decreased compared with Prdx6(+/+) LECs. Among the cytoskeleton associated proteins which were highly expressed in Prdx6-deficient LECs was tropomyosin (Tm) 2beta. Protein blot and real-time PCR validated dramatic increase of Tm2beta and Tm1alpha expression in these cells. Importantly, Prdx6(+/+) LECs showed similar pattern of Tm2beta protein expression following TGFbeta or H(2)O(2) treatment. An extrinsic supply of PRDX6 could restore the Tm2beta expression, demonstrating that PRDX6 may attenuate adverse signaling in cells and thereby maintains cellular homeostasis. By exploring redox-proteomic (Prdx6(-/-)) and characterization and identification of the abnormally expressed proteins, and their attenuation by PRDX6 delivery should provide a base to develop a novel therapeutic interventions to postponing ROS-mediated abnormal signaling deleterious to cells or tissues.
------>tmu_sno=None
------>sno=3638
------>authors2=None
------>authors3=None
------>authors4=None
------>authors5=None
------>authors6=None
------>authors6_c=None
------>authors=Deng, W.P., Hwang, J.J., Lin, G.L., Ngo, Q.H.
------>delete_flag=0
------>SCI_JNo=None
------>authors2_c=None
------>publish_area=None
------>updateTitle=Protein Expression Profiling of Lens Epithelial Cells from Prdx6-depleted Mice and Their Vulnerability to UV Radiation Exposure.
------>language=2
------>check_flag=
------>submit_date=
------>country=None
------>no=3
------>patent_SDate=None
------>update_bywho=
------>publish_year=1998
------>submit_flag=
------>publish_month=None
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z