Taipei Medical University

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
Uang Y-S
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------>journal_name=Journal of Chromatography B: Biomedical Applications
------>paper_name=Determination of mephenoxalone in human plasma sample by high-performance liquid chromatography-fluorescence detection
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------>fullAbstract=A simple and sensitive high-performance liquid chromatographic method involving fluorescence detection was developed for the determination of mephenoxalone in human plasma. A Cosmosil 5C18-MS column (250 mm x 4.6 mm I.D., 5 microm) was used as stationary phase and the mobile phase consisted of water-acetic acid-acetonitrile (200:1:300) at a flow-rate of 1.0 ml/min. The fluorescence absorbance was monitored at 280 nm for excitation wavelength and 310 nm for emission wavelength. Temperature control was kept at 40 degrees C for the column. The limit of quantitation achieved was 10 ng/ml, and the standard curve was found to be linear in the concentration range of 10-10,000 ng/ml. Under these analytical conditions, a sufficient mephenoxalone plasma concentration profile could be obtained for pharmacokinetic study.
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------>authors2=Chen I-K
------>authors3=Wang L-H
------>authors4=Hsu K-Y
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------>authors=Uang Y-S
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------>updateTitle=Determination of mephenoxalone in human plasma sample by high-performance liquid chromatography-fluorescence detection.
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------>publish_year=2001
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A B C D E F G H I J K L M N O P Q R S T U V W X Y Z