| Sawade S, Kokeguchi S, Miyamoto M, Sawada K, Fujimoto C, Wataki T, Hirosue M, Shimizu A, Chou HH, Kurihara H, Arai H, Takashiba S and Murayama Y |
------>authors3_c=None ------>paper_class1=1 ------>Impact_Factor=None ------>paper_class3=1 ------>paper_class2=1 ------>vol=40 ------>confirm_bywho=seanlee ------>insert_bywho=??? ------>Jurnal_Rank=None ------>authors4_c=None ------>comm_author=0 ------>patent_EDate=None ------>authors5_c=None ------>publish_day=None ------>paper_class2Letter=None ------>page2=485 ------>medlineContent= ------>unit=F0100 ------>insert_date=19991209 ------>iam=7 ------>update_date= ------>author=??? ------>change_event=6 ------>ISSN=None ------>authors_c=None ------>score=49 ------>journal_name=????????? ------>paper_name=Identification of periodontal bacteria using DNA probes based on bacterial 16s ribosomal RNA gene. ------>confirm_date=20060213 ------>tch_id=086008 ------>pmid=8804904 ------>page1=477 ------>fullAbstract=The aim of this study was to develop a PCR reaction specific to Actinobacillus actinomycetemcomitans, which targets a widely conserved gene of this bacterium. Two sets of primers were designed based on published sequences of the 16S rRNA of several microorganisms. The first set amplifies a major part of the 16S small subunit rRNA gene of several strains of bacteria commonly found in the periodontal pocket. This reaction produced a 1306 bp-long product and served as a positive control. The second set was specific to A. actinomycetemcomitans and produced a 449 bp-long product. H. aphrophilus and E. coli yielded positive results with the control primers and negative results with the A. actinomycetemcomitans-specific primers. DNA-DNA hybridization was used to validate the identify of the amplified sequences. B. cereus, which is a common contaminator in the laboratory, and human DNA did not generate PCR products in either reaction. The developed primers seen useful for the identification of A. actinomycetemcomitans strains. ------>tmu_sno=None ------>sno=460 ------>authors2=None ------>authors3=None ------>authors4=None ------>authors5=None ------>authors6=None ------>authors6_c=None ------>authors=Sawade S, Kokeguchi S, Miyamoto M, Sawada K, Fujimoto C, Wataki T, Hirosue M, Shimizu A, Chou HH, Kurihara H, Arai H, Takashiba S and Murayama Y ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c=None ------>publish_area=None ------>updateTitle=PCR primers for the amplification of the 16S rRNA gene of oral bacteria and for the specific identification of Actinobacillus actinomycetemcomitans. ------>language= ------>check_flag=0 ------>submit_date= ------>country=None ------>no=4 ------>patent_SDate=None ------>update_bywho= ------>publish_year=1998 ------>submit_flag= ------>publish_month=None |