Taipei Medical University

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
K. Y. Hwa* and K. H. Khoo*
------>authors3_c=None
------>paper_class1=1
------>Impact_Factor=None
------>paper_class3=2
------>paper_class2=1
------>vol=111
------>confirm_bywho=1
------>insert_bywho=kyhwa
------>Jurnal_Rank=None
------>authors4_c=None
------>comm_author=1
------>patent_EDate=None
------>authors5_c=None
------>publish_day=None
------>paper_class2Letter=None
------>page2=184
------>medlineContent=
------>unit=000
------>insert_date=20020310
------>iam=1
------>update_date=
------>author=???
------>change_event=2
------>ISSN=None
------>authors_c=None
------>score=500
------>journal_name=Mol Biochem Parasitol
------>paper_name=Structural analysis of the asparagine-linked glycans from the procyclic Trypanosoma brucei and its glycosylation mutants resistant to Concanavalin A killing
------>confirm_date=
------>tch_id=090057
------>pmid=11087927
------>page1=173
------>fullAbstract=The variant surface glycoprotein of the bloodstream form of Trypanosoma brucei is known to be glycosylated with a range of structures including high mannose and complex types. In contrast, glycosylation in the procyclic form of the parasite appears to be restricted to a single Man(5)GlcNAc(2) structure, as found on its procyclin. To gain a better insight into the developmentally regulated glycosylation pattern, we have structurally defined the full range of N-linked glycans made by the procyclic trypanosomes, as well as two previously described glycosylation mutants generated under Con A selection. It was found that the wild type procyclic cells could synthesize a full range of high mannose type structures from Man(5)GlcNAc(2) to Man(9)GlcNAc(2), with Man(5)GlcNAc(2) as the major component. In contrast, the two mutants mainly synthesized a truncated Man(4)GlcNAc(2) structure, Man alpha 1-3Man alpha 1-6(Man alpha1-3)Man be ta 1-4 GlcNAc beta 1-4GlcNAc, a significant portion of which was further extended by a single GlcNAc to form GlcNAc-Man(4)GlcNAc(2) and a single N-acetyllactosamine unit at the 3-arm position to form Gal beta 1-4GlcNAc beta 1-2Man alpha 1-3(Man al pha 1- 3Man alpha 1-6)Man beta 1-4G lcNAc beta 1-4GlcNAc. The results suggest that the procyclic trypanosomes could be induced by Con A selection to synthesize limited hybrid type structures, but in general do not further process their N-linked glycans into multiantennary complex types as the blood stream forms do.
------>tmu_sno=None
------>sno=4857
------>authors2=None
------>authors3=None
------>authors4=None
------>authors5=None
------>authors6=None
------>authors6_c=None
------>authors=K. Y. Hwa* and K. H. Khoo*
------>delete_flag=0
------>SCI_JNo=None
------>authors2_c=None
------>publish_area=None
------>updateTitle=Structural analysis of the asparagine-linked glycans from the procyclic Trypanosoma brucei and its glycosylation mutants resistant to Concanavalin A killing.
------>language=2
------>check_flag=
------>submit_date=
------>country=None
------>no=1
------>patent_SDate=None
------>update_bywho=
------>publish_year=2000
------>submit_flag=
------>publish_month=None
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z