K. Y. Hwa* and K. H. Khoo* |
------>authors3_c=None ------>paper_class1=1 ------>Impact_Factor=None ------>paper_class3=2 ------>paper_class2=1 ------>vol=111 ------>confirm_bywho=1 ------>insert_bywho=kyhwa ------>Jurnal_Rank=None ------>authors4_c=None ------>comm_author=1 ------>patent_EDate=None ------>authors5_c=None ------>publish_day=None ------>paper_class2Letter=None ------>page2=184 ------>medlineContent= ------>unit=000 ------>insert_date=20020310 ------>iam=1 ------>update_date= ------>author=??? ------>change_event=2 ------>ISSN=None ------>authors_c=None ------>score=500 ------>journal_name=Mol Biochem Parasitol ------>paper_name=Structural analysis of the asparagine-linked glycans from the procyclic Trypanosoma brucei and its glycosylation mutants resistant to Concanavalin A killing ------>confirm_date= ------>tch_id=090057 ------>pmid=11087927 ------>page1=173 ------>fullAbstract=The variant surface glycoprotein of the bloodstream form of Trypanosoma brucei is known to be glycosylated with a range of structures including high mannose and complex types. In contrast, glycosylation in the procyclic form of the parasite appears to be restricted to a single Man(5)GlcNAc(2) structure, as found on its procyclin. To gain a better insight into the developmentally regulated glycosylation pattern, we have structurally defined the full range of N-linked glycans made by the procyclic trypanosomes, as well as two previously described glycosylation mutants generated under Con A selection. It was found that the wild type procyclic cells could synthesize a full range of high mannose type structures from Man(5)GlcNAc(2) to Man(9)GlcNAc(2), with Man(5)GlcNAc(2) as the major component. In contrast, the two mutants mainly synthesized a truncated Man(4)GlcNAc(2) structure, Man alpha 1-3Man alpha 1-6(Man alpha1-3)Man be ta 1-4 GlcNAc beta 1-4GlcNAc, a significant portion of which was further extended by a single GlcNAc to form GlcNAc-Man(4)GlcNAc(2) and a single N-acetyllactosamine unit at the 3-arm position to form Gal beta 1-4GlcNAc beta 1-2Man alpha 1-3(Man al pha 1- 3Man alpha 1-6)Man beta 1-4G lcNAc beta 1-4GlcNAc. The results suggest that the procyclic trypanosomes could be induced by Con A selection to synthesize limited hybrid type structures, but in general do not further process their N-linked glycans into multiantennary complex types as the blood stream forms do. ------>tmu_sno=None ------>sno=4857 ------>authors2=None ------>authors3=None ------>authors4=None ------>authors5=None ------>authors6=None ------>authors6_c=None ------>authors=K. Y. Hwa* and K. H. Khoo* ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c=None ------>publish_area=None ------>updateTitle=Structural analysis of the asparagine-linked glycans from the procyclic Trypanosoma brucei and its glycosylation mutants resistant to Concanavalin A killing. ------>language=2 ------>check_flag= ------>submit_date= ------>country=None ------>no=1 ------>patent_SDate=None ------>update_bywho= ------>publish_year=2000 ------>submit_flag= ------>publish_month=None |