| Dugan TA |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=5.854 ------>paper_class3=2 ------>paper_class2=1 ------>vol=278 ------>confirm_bywho=wpdeng ------>insert_bywho=vyang ------>Jurnal_Rank=14.6 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=13662 ------>medlineContent= ------>unit=F0700 ------>insert_date=20030319 ------>iam=2 ------>update_date= ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=500 ------>journal_name=Journal of Biological Chemistry ------>paper_name=Decorin Binds Fibrinogen in a Zn2+-dependent interaction ------>confirm_date=20081128 ------>tch_id=090071 ------>pmid=12582160 ------>page1=13655 ------>fullAbstract=We have previously shown that decorin, a member of the small leucine-rich proteoglycan family of extracellular matrix proteoglycans/glycoproteins is a Zn(2+) metalloprotein at physiological Zn(2+) concentrations (Yang, V. W-C., LaBrenz, S. R., Rosenberg, L. C., McQuillan, D., and Hook, M. (1999) J. Biol. Chem. 274, 12454-12460). We now report that the decorin proteoglycan binds fibrinogen in the presence of Zn(2+). The fibrinogen-binding site is located in the N-terminal domain of the decorin core protein and a 45-amino acid peptide representing this domain binds to the fibrinogen D fragment with an apparent K(D) of 1.7 x 10(-6) m, as determined from fluorescence polarization data. Furthermore, we show that Zn(2+) promotes the self-association of decorin. The N-terminal domain of the core protein also mediates this activity. The results of solid-phase binding assays and gel filtration chromatography suggest that the N-terminal domain of decorin, when present at low micromolar concentrations, forms an oligomer in a Zn(2+)-dependent manner. Thus, Zn(2+) appears to play a pivotal role in the interactions and biological function of decorin. ------>tmu_sno=None ------>sno=6355 ------>authors2=Yang WCV ------>authors3=McQuillan JD ------>authors4=Höök M ------>authors5= ------>authors6= ------>authors6_c= ------>authors=Dugan TA ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Decorin binds fibrinogen in a Zn2+-dependent interaction. ------>language=2 ------>check_flag= ------>submit_date= ------>country=None ------>no=16 ------>patent_SDate=None ------>update_bywho= ------>publish_year=2003 ------>submit_flag= ------>publish_month=1 |