| Shih YH, Wu SL, Chiou WF, Ku HH, Ko TL, Fu YS |
------>authors3_c=None ------>paper_class1=1 ------>Impact_Factor=None ------>paper_class3=2 ------>paper_class2=1 ------>vol=13 ------>confirm_bywho=thfong ------>insert_bywho=cate819b ------>Jurnal_Rank=None ------>authors4_c=None ------>comm_author= ------>patent_EDate=None ------>authors5_c=None ------>publish_day=None ------>paper_class2Letter=None ------>page2=519 ------>medlineContent= ------>unit=E0107 ------>insert_date=20030425 ------>iam=5 ------>update_date= ------>author=??? ------>change_event=6 ------>ISSN=None ------>authors_c=None ------>score=500 ------>journal_name=Neuroreport ------>paper_name=Protective effects of tetramethylpyrazine on kainate-induced excitotoxicity in hippocampal culture ------>confirm_date=20060210 ------>tch_id=081005 ------>pmid=11930173 ------>page1=515 ------>fullAbstract=Tetramethylpyrazine (TMP) is the major component extracted from the Chinese herb, Chuanxiong. This study focuses on the protective effect of tetramethylpyrazine in kainate-induced excitotoxicity in rat hippocampus. Primary neuronal cultures raised from cells isolated from the hippocampi of 7-day old rats were treated with kainate (75-450 microM) for 12, 24, and 48 h. Our results revealed that kainate induced neuronal damage in a dose- and time-dependent manner, reaching maximal damage at 150 microM and 24 h and persisted for higher doses and 48 h. In addition, 1 h of kainate (150 microM) treatment led to significant generation of free radicals and reduction of mitochondrial membrane potential (MMP) which persisted for > or = 4 h on continued exposure. Ten minutes pretreatment with 1 or 5 microM tetramethylpyrazine dose dependently and significantly attenuated the kainate-induced damage. Taken together, the results suggest that multiple mechanisms including protection of mitochondria, decrease in free radical generation and scavenging of free radicals might be involved in TMP~s protection against kainate induced cell toxicity. ------>tmu_sno=None ------>sno=7145 ------>authors2=None ------>authors3=None ------>authors4=None ------>authors5=None ------>authors6=None ------>authors6_c=None ------>authors=Shih YH, Wu SL, Chiou WF, Ku HH, Ko TL, Fu YS ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c=None ------>publish_area=None ------>updateTitle=Protective effects of tetramethylpyrazine on kainate-induced excitotoxicity in hippocampal culture. ------>language=2 ------>check_flag= ------>submit_date= ------>country=None ------>no=4 ------>patent_SDate=None ------>update_bywho= ------>publish_year=2002 ------>submit_flag= ------>publish_month=None |