| Yang LL |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=2.532 ------>paper_class3=2 ------>paper_class2=1 ------>vol=51 ------>confirm_bywho=amel ------>insert_bywho=llyang ------>Jurnal_Rank=2.9 ------>authors4_c= ------>comm_author= ------>patent_EDate=None ------>authors5_c= ------>publish_day=7 ------>paper_class2Letter=None ------>page2=2979 ------>medlineContent= ------>unit=G0100 ------>insert_date=20030512 ------>iam=1 ------>update_date= ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=500 ------>journal_name=J Agric Food Chem. ------>paper_name=Antitumor principle constituents of Myrica rubra Var. acuminata ------>confirm_date=20081217 ------>tch_id=058001 ------>pmid=12720379 ------>page1=2974 ------>fullAbstract=Myrica rubra var. acuminata is a native shrub widely distributed and used as folk medicine in Taiwan for stomach disorders and diarrhea. Column chromatography combined with cytotoxic bioassay-guided fractionation was performed to isolate the antitumor principles from fresh leaves of M. rubravar. acuminata. The 20% MeOH eluate fraction of M. rubra var. acuminata inhibited the viability of HeLa and P-388 cells in an in vitro assay and an in vivo P-388 tumor-bearing CDF(1) mouse model. The percent increase in life span (%ILS) of 20% MeOH eluate fraction was greater than 125%. (-)-Epigallocatechin 3-O-gallate (1) and prodelphinidin A-2,3~-O-gallate (2) were isolated from D-20 as the antitumor principle components. Both compounds can inhibit the growth of HeLa cells, but 1 had lower cytotoxic effects in normal cervical fibroblasts than did 2. Moreover, pretreatment with a caspase-3 specific inhibitor prevented 1- and 2-induced poly(ADP-ribose) polymerase cleavage. In view of these results, we suggest that 1 and 2 can induce apoptosis in HeLa cells and that activation of caspase-3 may provide a mechanistic explanation for their cytotoxic effects. Therefore, we suggest that the 20% MeOH eluate fraction extract is good for health and that M. rubra var. acuminata is an economically valuable plant. ------>tmu_sno=None ------>sno=7298 ------>authors2=Chang CC ------>authors3=Chen LG ------>authors4=Wang CC ------>authors5= ------>authors6= ------>authors6_c= ------>authors=Yang LL ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Antitumor principle constituents of Myrica rubra Var. acuminata. ------>language=2 ------>check_flag= ------>submit_date= ------>country=None ------>no=10 ------>patent_SDate=None ------>update_bywho= ------>publish_year=2003 ------>submit_flag= ------>publish_month=5 |