| Ling TY, Huang YH, Lai MC, Huang SS and Huang JS |
------>authors3_c=None ------>paper_class1=1 ------>Impact_Factor=6.820 ------>paper_class3=2 ------>paper_class2=1 ------>vol=17 ------>confirm_bywho=None ------>insert_bywho=rita1204 ------>Jurnal_Rank=3.1 ------>authors4_c=None ------>comm_author= ------>patent_EDate=None ------>authors5_c=None ------>publish_day=1 ------>paper_class2Letter=None ------>page2=1561 ------>medlineContent= ------>unit=E0103 ------>insert_date=20030916 ------>iam=2 ------>update_date= ------>author=??? ------>change_event=6 ------>ISSN= ------>authors_c=None ------>score=500 ------>journal_name=FASEB J ------>paper_name=Fatty acids modulate transforming growth factor-beta activity and plasma clearance ------>confirm_date=None ------>tch_id=090143 ------>pmid=12824279 ------>page1=1559 ------>fullAbstract=The activity and plasma clearance of transforming growth factor (TGF)-beta are known to be regulated by activated alpha2-macroglobulin (alpha2M*). This has been implicated in pathophysiological processes, but no small molecule compounds have been reported to modulate TGF-beta activity by affecting the interaction of TGF-beta and alpha2M*. Here, we demonstrate that fatty acids are capable of inhibiting complex formation of TGF-beta isoforms and alpha2M* as demonstrated by nondenaturing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This is dependent on carbon chain length (C20, C18, C16, C14 > C12 > C10), degree of unsaturation (polyunsaturated > saturated), and TGF-beta isoforms (TGF-beta1 > TGF-beta2 > TGF-beta3). Arachidonic acid, which is one of the most potent inhibitors, is also capable of dissociating TGF-beta-alpha2M* complexes, but higher concentrations are required. Arachidonic acid appears to inhibit TGF-beta-alpha2M* complex formation by binding specifically to alpha2M* as demonstrated by gel filtration chromatography. Arachidonic acid reverses the inhibitory effect of alpha2M* on TGF-beta binding, TGF-beta-induced growth inhibition, and TGF-beta-induced transcriptional activation in mink lung epithelial cells and affects plasma clearance of TGF-beta-alpha2M* complexes in mice. These results show that fatty acids are effective modulators of TGF-beta activity and plasma clearance and may be useful in treating human diseases through their effects on the interaction of TGF-beta and alpha2M*. ------>tmu_sno=None ------>sno=7560 ------>authors2= ------>authors3= ------>authors4= ------>authors5= ------>authors6= ------>authors6_c=None ------>authors=Ling TY, Huang YH, Lai MC, Huang SS and Huang JS ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c=None ------>publish_area=0 ------>updateTitle=Fatty acids modulate transforming growth factor-beta activity and plasma clearance. ------>language=2 ------>check_flag= ------>submit_date= ------>country=None ------>no=11 ------>patent_SDate=None ------>update_bywho= ------>publish_year=2003 ------>submit_flag= ------>publish_month=8 |