| Huang SS, Tang FM, Huang YH, Liu IH, Hsu SC, Chen ST and Huang JS |
------>authors3_c=None ------>paper_class1=1 ------>Impact_Factor=6.355 ------>paper_class3=2 ------>paper_class2=1 ------>vol=278 ------>confirm_bywho=None ------>insert_bywho=rita1204 ------>Jurnal_Rank=11.9 ------>authors4_c=None ------>comm_author= ------>patent_EDate=None ------>authors5_c=None ------>publish_day=1 ------>paper_class2Letter=None ------>page2=43869 ------>medlineContent= ------>unit=E0103 ------>insert_date=20030916 ------>iam=3 ------>update_date= ------>author=??? ------>change_event=6 ------>ISSN= ------>authors_c=None ------>score=500 ------>journal_name=J. Biol. Chem. ------>paper_name=Cloning, expression, characterization and role in autocrine cell growth of cell surface retention sequence binding protein-1. ------>confirm_date=None ------>tch_id=090143 ------>pmid=12912978 ------>page1=43855 ------>fullAbstract=Cell surface retention sequence binding protein-1 (CRSBP-1) is a cell surface binding protein for the cell surface retention sequence (CRS) motif of the v-sis gene product (platelet-derived growth factor-BB). It has been shown to be responsible for cell surface retention of the v-sis gene product in v-sis-transformed cells (fibroblasts) and has been hypothesized to play a role in autocrine growth and transformation of these cells. Here we demonstrate that the CRSBP-1 cDNA cloned from bovine liver libraries encodes a 322-residue type I membrane protein containing a 23-residue signal peptide, a 215-residue cell surface domain, a 21-residue transmembrane domain, and a 63-residue cytoplasmic domain. CRSBP-1 expressed in transfected cells is an approximately 120-kDa disulfide-linked homodimeric glycoprotein and exhibits dual ligand (CRS-containing growth regulators (v-sis gene product and insulin-like growth factor binding protein-3, IGFBP-3) and hyaluronic acid) binding activity. CRSBP-1 overexpression (by stable transfection of cells with CRSBP-1 cDNA) enhances autocrine loop signaling, cell growth, and tumorigenicity (in mice) of v-sis-transformed cells. CRSBP-1 expression also enhances autocrine cell growth mediated by IGFBP-3 in human lung carcinoma cells (H1299 cells), which express very little, if any, endogenous CRSBP-1 and exhibits a mitogenic response to exogenous IGFBP-3, stably transfected with IGFBP-3 cDNA. However, CRSBP-1 overexpression does not affect growth of normal and transformed cells that do not produce these CRS-containing growth regulators. These results suggest that CRSBP-1 plays a role in autocrine regulation of cell growth mediated by growth regulators containing CRS. ------>tmu_sno=None ------>sno=7561 ------>authors2= ------>authors3= ------>authors4= ------>authors5= ------>authors6= ------>authors6_c=None ------>authors=Huang SS, Tang FM, Huang YH, Liu IH, Hsu SC, Chen ST and Huang JS ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c=None ------>publish_area=0 ------>updateTitle=Cloning, expression, characterization, and role in autocrine cell growth of cell surface retention sequence binding protein-1. ------>language=2 ------>check_flag= ------>submit_date= ------>country=None ------>no=44 ------>patent_SDate=None ------>update_bywho= ------>publish_year=2003 ------>submit_flag= ------>publish_month=10 |