| Huang SS, Ling TY, Tseng WF, Huang YH, Tang FM, Leal SM and Huang JS |
------>authors3_c=None ------>paper_class1=1 ------>Impact_Factor=6.820 ------>paper_class3=2 ------>paper_class2=1 ------>vol=17 ------>confirm_bywho=None ------>insert_bywho=rita1204 ------>Jurnal_Rank=3.1 ------>authors4_c=None ------>comm_author= ------>patent_EDate=None ------>authors5_c=None ------>publish_day=1 ------>paper_class2Letter=None ------>page2=2081 ------>medlineContent= ------>unit=E0103 ------>insert_date=20031006 ------>iam=4 ------>update_date= ------>author=??? ------>change_event=6 ------>ISSN= ------>authors_c=None ------>score=486 ------>journal_name=FASEB J ------>paper_name=Cellular growth inhibition by IGFBP-3 and TGF-beta1 required LRP-1. ------>confirm_date=None ------>tch_id=090143 ------>pmid=14597676 ------>page1=2068 ------>fullAbstract=The type V TGF-beta receptor (TbetaR-V)/IGFBP-3 receptor mediates the IGF-independent growth inhibition induced by IGFBP-3. It also mediates the growth inhibitory response to TGF-beta1 in concert with other TGF-beta receptor types, and its loss may contribute to the malignant phenotype of human carcinoma cells. Here we demonstrate that TbetaR-V is identical to LRP-1/alpha2M receptor as shown by MALDI-TOF analysis of tryptic peptides of TbetaR-V purified from bovine liver. In addition, 125I-IGFBP-3 affinity-labeled TbetaR-V in Mv1Lu cells is immunoprecipitated by antibodies to LRP-1 and TbetaR-V. RAP, an LRP-1 antagonist, inhibits binding of 125I-TGF-beta1 and 125I-IGFBP-3 to TbetaR-V and diminishes IGFBP-3-induced growth inhibition in Mv1Lu cells. Absent or low levels of LRP-1, as with TbetaR-V, have been linked to the malignant phenotype of carcinoma cells. Mutagenized Mv1Lu cells selected for reduced expression of LRP-1 have an attenuated growth inhibitory response to TGF-beta1 and IGFBP-3. LRP-1-deficient mouse embryonic fibroblasts lack a growth inhibitory response to TGF-beta1 and IGFBP-3. On the other hand, stable transfection of H1299 human lung carcinoma cells with LRP-1 cDNA restores the growth inhibitory response. These results suggest that the LRP-1/TbetaR-V/IGFBP-3 receptor is required for the growth inhibitory response to IGFBP-3 and TGF-beta1. ------>tmu_sno=None ------>sno=7577 ------>authors2= ------>authors3= ------>authors4= ------>authors5= ------>authors6= ------>authors6_c=None ------>authors=Huang SS, Ling TY, Tseng WF, Huang YH, Tang FM, Leal SM and Huang JS ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c=None ------>publish_area=0 ------>updateTitle=Cellular growth inhibition by IGFBP-3 and TGF-beta1 requires LRP-1. ------>language=2 ------>check_flag= ------>submit_date= ------>country=None ------>no=14 ------>patent_SDate=None ------>update_bywho= ------>publish_year=2003 ------>submit_flag= ------>publish_month=11 |