| Chang RC, Chen CC and ShawJF |
------>authors3_c=None ------>paper_class1=1 ------>Impact_Factor=None ------>paper_class3=2 ------>paper_class2=1 ------>vol=229 ------>confirm_bywho=tzengcr ------>insert_bywho=??? ------>Jurnal_Rank=None ------>authors4_c=None ------>comm_author=0 ------>patent_EDate=None ------>authors5_c=None ------>publish_day=None ------>paper_class2Letter=None ------>page2=10 ------>medlineContent= ------>unit=G0200 ------>insert_date=19991209 ------>iam=3 ------>update_date= ------>author=??? ------>change_event=5 ------>ISSN=None ------>authors_c=None ------>score=500 ------>journal_name=Biochem. Biophys. Res. Commun. ------>paper_name=Studying the active site pocket of Staphylococcus hyicus lipase by site-directed mutagenesis. ------>confirm_date=20030509 ------>tch_id=086069 ------>pmid=8954075 ------>page1=6 ------>fullAbstract=Site-directed mutagenesis of a previously constructed, recombinant Staphylococcus hyicus lipase (49 kDa) showed that Val363 played a role in catalysis and substrate-binding. In comparison with wild type enzyme, the 64% and 89% decrease in the catalytic efficiency (kcat/Km) of the V363N and V363A enzymes, respectively, were largely caused by a 3.5- and 5.5-fold increase in the substrate-binding affinity (Km), respectively. In comparison with wild type enzyme, a G371A enzyme showed a 40% decrease in the Km, suggesting that G371 was important for substrate-binding specificity. Site-directed mutagenesis of the active site Asp559 revealed that in comparison with wild type enzyme, a D559E enzyme exhibited a 47% decrease in the kcat/Km but a twofold increase in the Km for p-nitrophenyl butyrate, suggesting that Asp-559, a component of the catalytic triad, was involved in substrate-specificity. ------>tmu_sno=None ------>sno=81 ------>authors2=None ------>authors3=None ------>authors4=None ------>authors5=None ------>authors6=None ------>authors6_c=None ------>authors=Chang RC, Chen CC and ShawJF ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c=None ------>publish_area=None ------>updateTitle=Studying the active site pocket of Staphylococcus hyicus lipase by site-directed mutagenesis. ------>language= ------>check_flag=0 ------>submit_date= ------>country=None ------>no=1 ------>patent_SDate=None ------>update_bywho= ------>publish_year=1996 ------>submit_flag= ------>publish_month=None |