| Chang RC, Chen JC and ShawJF |
------>authors3_c=None ------>paper_class1=1 ------>Impact_Factor=None ------>paper_class3=2 ------>paper_class2=1 ------>vol=228 ------>confirm_bywho=tzengcr ------>insert_bywho=??? ------>Jurnal_Rank=None ------>authors4_c=None ------>comm_author=0 ------>patent_EDate=None ------>authors5_c=None ------>publish_day=None ------>paper_class2Letter=None ------>page2=779 ------>medlineContent= ------>unit=G0200 ------>insert_date=19991209 ------>iam=3 ------>update_date= ------>author=??? ------>change_event=5 ------>ISSN=None ------>authors_c=None ------>score=500 ------>journal_name=Biochem. Biophys. Res. Commun. ------>paper_name=Facile purification of highly active recombinant Staphylococcus hyicus lipase fragment and characterization of a putative lid region. ------>confirm_date=20030509 ------>tch_id=086069 ------>pmid=8941352 ------>page1=774 ------>fullAbstract=A fragment of Staphylococcus hyicus lipase gene (Ala248 to Ala640) was inserted into plasmid pET20(b+). The resulting His-tagged recombinant protein (49 kDa) was overexpressed in Escherichia coli BL21(DE3) as an highly active lipase and was purified by nickel-coupled resin. Site-directed mutagenesis showed that in comparison with wild type enzyme, the L326F and L326A enzymes showed a 3.4 and 5 fold increase in the K(m), respectively, but only a 44% and a 64% decrease in the kcat/K(m), respectively, suggesting that Leu326 of the putative lid participated in substrate-binding. ------>tmu_sno=None ------>sno=82 ------>authors2=None ------>authors3=None ------>authors4=None ------>authors5=None ------>authors6=None ------>authors6_c=None ------>authors=Chang RC, Chen JC and ShawJF ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c=None ------>publish_area=None ------>updateTitle=Facile purification of highly active recombinant Staphylococcus hyicus lipase fragment and characterization of a putative lid region. ------>language= ------>check_flag=0 ------>submit_date= ------>country=None ------>no=3 ------>patent_SDate=None ------>update_bywho= ------>publish_year=1996 ------>submit_flag= ------>publish_month=None |