| Ho JD |
------>authors3_c=None ------>paper_class1=1 ------>Impact_Factor=None ------>paper_class3=2 ------>paper_class2=1 ------>vol=121 ------>confirm_bywho=1 ------>insert_bywho=jauder ------>Jurnal_Rank=None ------>authors4_c=None ------>comm_author= ------>patent_EDate=None ------>authors5_c=None ------>publish_day=None ------>paper_class2Letter=None ------>page2=1429 ------>medlineContent= ------>unit=000 ------>insert_date=20040419 ------>iam=1 ------>update_date=None ------>author=??? ------>change_event=2 ------>ISSN=None ------>authors_c=None ------>score=500 ------>journal_name=Arch Ophthalmol ------>paper_name=Cytotoxicity of Indocyanine Green on Retinal Pigment Epithelium: Implications for Macular Hole Surgery ------>confirm_date=None ------>tch_id=093018 ------>pmid=14557178 ------>page1=1423 ------>fullAbstract=OBJECTIVE: To evaluate the potential cytotoxic effects of indocyanine green (ICG) on cultured human retinal pigment epithelium (RPE) and the resultant implications for macular hole surgery. METHODS: Human RPE cells were exposed to ICG in concentrations from 0.001 to 5 mg/mL. The exposure duration ranged from 5 minutes to 3 hours. Light microscopy, MTS viability assay, and calcein AM-ethidium homodimer 1 staining were used to evaluate the cytotoxic effects of ICG. RESULTS: The RPE cells incubated with up to 5 mg/mL of ICG for 5 minutes or less exhibited no morphologic change and no significant decrease in dehydrogenase activity. When RPE cells were exposed to 5 mg/mL of ICG for 10 minutes, 1 mg/mL of ICG for 20 minutes, or 0.01 mg/mL of ICG for 3 hours, cell morphologic features were altered, mitochondrial dehydrogenase activity decreased, and some cells were necrotic. CONCLUSIONS: Indocyanine green caused cytotoxicity in cultured human RPE in a dose- and time-dependent manner. Cell death occurred by necrosis. CLINICAL RELEVANCE: Exposure of RPE cells to ICG concentrations up to 5 mg/mL for 5 minutes or less was not injurious; prolonged exposure to a low ICG concentration was toxic. Since ICG may be retained in the vitreous cavity for a lengthy period, thorough washout of ICG during macular hole surgery is required. ------>tmu_sno=None ------>sno=8486 ------>authors2=Tsai RJF ------>authors3=Chen SN ------>authors4=Chen HC ------>authors5= ------>authors6= ------>authors6_c=None ------>authors=Ho JD ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c=None ------>publish_area=None ------>updateTitle=Cytotoxicity of indocyanine green on retinal pigment epithelium: implications for macular hole surgery. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2003 ------>submit_flag=None ------>publish_month=None |