| Wehr T, Rodrigues-Diaz R. and Liu CM |
------>authors3_c=None ------>paper_class1=1 ------>Impact_Factor=None ------>paper_class3=2 ------>paper_class2=1 ------>vol=38 ------>confirm_bywho=yangyuan ------>insert_bywho=??? ------>Jurnal_Rank=None ------>authors4_c=None ------>comm_author=0 ------>patent_EDate=None ------>authors5_c=None ------>publish_day=None ------>paper_class2Letter=None ------>page2=352 ------>medlineContent= ------>unit=E0300 ------>insert_date=19991209 ------>iam=3 ------>update_date= ------>author=??? ------>change_event=5 ------>ISSN=None ------>authors_c=None ------>score=41 ------>journal_name=Advances in Chromatogr ------>paper_name=Capillary electrophoresis of protein. ------>confirm_date=20000518 ------>tch_id=087002 ------>pmid=19899081 ------>page1=238 ------>fullAbstract=Hepatocellular carcinoma (HCC) is one of the deadliest cancers with few treatment options. It is a hypervascular tumor in which angiogenesis plays a critical role in its progression. Tumor capillary endothelial cells in HCC (TECs) are known to originate from liver sinusoid endothelial cells (SECs) which then go through a capillarization process to become morphologically as well as functionally different TECs. In this work, we investigated proteins differentially expressed between freshly isolated TECs and SECs from well-formed rat HCC using two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) coupled with MALDI-TOF/TOF MS. Thirty-eight unique proteins were identified to be differentially expressed more than 2-fold between the two endothelial cell types. Amongst the differentially expressed proteins, two novel endothelial markers, EH domain-containing protein 3 (EHD3) and Galectin-3, were confirmed by Western blot and immunohistochemistry in both rat and human HCC samples. We showed that EHD3 is significantly down-regulated in TECs, but Galectin-3 is up-regulated. We propose possible roles of these two proteins in tumor vessel development in HCC. ------>tmu_sno=None ------>sno=875 ------>authors2=None ------>authors3=None ------>authors4=None ------>authors5=None ------>authors6=None ------>authors6_c=None ------>authors=Wehr T, Rodrigues-Diaz R. and Liu CM ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c=None ------>publish_area=None ------>updateTitle=Identification of proteins differentially expressed between capillary endothelial cells of hepatocellular carcinoma and normal liver in an orthotopic rat tumor model using 2D-DIGE. ------>language= ------>check_flag=0 ------>submit_date= ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho= ------>publish_year=1997 ------>submit_flag= ------>publish_month=None |