| Chang CJ |
------>authors3_c= ------>paper_class1=1 ------>Impact_Factor=1.930 ------>paper_class3=2 ------>paper_class2=1 ------>vol=1042 ------>confirm_bywho=sihuang ------>insert_bywho=sihuang ------>Jurnal_Rank=13.7 ------>authors4_c= ------>comm_author=1 ------>patent_EDate=None ------>authors5_c= ------>publish_day=1 ------>paper_class2Letter=None ------>page2=302 ------>medlineContent= ------>unit=J0400 ------>insert_date=20040914 ------>iam=5 ------>update_date=None ------>author=??? ------>change_event=4 ------>ISSN= ------>authors_c= ------>score=427 ------>journal_name=Annals N Y Academy Science ------>paper_name=Effects of Glucose and alfa-Tocopherol on Low Density Lipoprotein (LDL) Oxidation and Glycation ------>confirm_date=20070901 ------>tch_id=085004 ------>pmid=15965075 ------>page1=294 ------>fullAbstract=Glycation of blood proteins is considered to be a major contributor to hyperglycemic complications in diabetes mellitus patients. In this study, we demonstrate the efficacy of alpha-tocopherol in reducing low-density lipoprotein (LDL) oxidation and glycation in vitro. Native LDL isolated from healthy subjects was exposed to various concentrations of glucose and malondialdehyde (MDA) with or without alpha-tocopherol enrichment for 7 days in sealed vacuum ampoules. The degree of glycation, copper-induced lag time, content of thiobarbituric acid-reactive substances (TBARS), and alpha-tocopherol levels in LDL were then assessed. LDL lag time was significantly reduced with high levels of glucose and MDA. Alpha-tocopherol enrichment dramatically inhibited the oxidation of LDL in the lag-time assay. However, the length of incubation time was inversely related to the LDL lag time. Longer incubation time resulted in shorter LDL lag time, with or without alpha-tocopherol enrichment. The level of TBARS associated with LDL oxidation was highest in native, MDA-supplemented, and high-glucose samples. The alpha-tocopherol levels were inversely related to glucose levels and incubation times. In conclusion, high-glucose concentrations heightened the oxidative susceptibility of LDL. Alpha-tocopherol enrichment reduced this trend and prevented LDL from undergoing architectural modification. ------>tmu_sno=None ------>sno=9697 ------>authors2=Hsieh RH ------>authors3=Wang HF ------>authors4=Chin MY ------>authors5=Huang SY ------>authors6= ------>authors6_c= ------>authors=Chang CJ ------>delete_flag=0 ------>SCI_JNo=None ------>authors2_c= ------>publish_area=0 ------>updateTitle=Effects of glucose and alpha-tocopherol on low-density lipoprotein oxidation and glycation. ------>language=2 ------>check_flag=None ------>submit_date=None ------>country=None ------>no= ------>patent_SDate=None ------>update_bywho=None ------>publish_year=2005 ------>submit_flag=None ------>publish_month=1 |